Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual counting.

@article{Cai2011OptimizedDC,
  title={Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual counting.},
  author={Zhongli Cai and Niladri Chattopadhyay and Wenchao Jessica Liu and Conrad E. Z. Chan and J. Pignol and Raymond M Reilly},
  journal={International journal of radiation biology},
  year={2011},
  volume={87 11},
  pages={1135-46}
}
PURPOSE To develop a digital method for counting colonies that highly replicates manual counting. MATERIALS AND METHODS Breast cancer cells were treated with trastuzumab-conjugated gold nanoparticles in combination with X-ray irradiation, (111)In labeled trastuzumab, or γ-radiation, followed by clonogenic assays. Colonies were counted manually or digitally using ImageJ software with customized macros. Key parameters, intensity threshold and minimum colony size, were optimized based on three… CONTINUE READING