Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: Comparison with manual counting

@article{Cai2011OptimizedDC,
  title={Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: Comparison with manual counting},
  author={Zhongli Cai and Niladri Chattopadhyay and Wenchao Jessica Liu and Conrad Chan and J. Pignol and Raymond M. Reilly},
  journal={International Journal of Radiation Biology},
  year={2011},
  volume={87},
  pages={1135 - 1146}
}
Abstract Purpose: To develop a digital method for counting colonies that highly replicates manual counting. Materials and methods: Breast cancer cells were treated with trastuzumab-conjugated gold nanoparticles in combination with X-ray irradiation, 111In labeled trastuzumab, or γ-radiation, followed by clonogenic assays. Colonies were counted manually or digitally using ImageJ software with customized macros. Key parameters, intensity threshold and minimum colony size, were optimized based on… 
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