Optimized co-solute paramagnetic relaxation enhancement for the rapid NMR analysis of a highly fibrillogenic peptide.

@article{Oktaviani2015OptimizedCP,
  title={Optimized co-solute paramagnetic relaxation enhancement for the rapid NMR analysis of a highly fibrillogenic peptide.},
  author={Nur Alia Oktaviani and Michael Wulff Ris\or and Young-ho Lee and Rik P. Megens and Djurre H. de Jong and Renee Otten and Ruud M. Scheek and Jan J Enghild and N. C. Nielsen and Takahisa Ikegami and Frans A. A. Mulder},
  journal={Journal of biomolecular NMR},
  year={2015},
  volume={62 2},
  pages={129-42}
}
Co-solute paramagnetic relaxation enhancement (PRE) is an attractive way to speed up data acquisition in NMR spectroscopy by shortening the T 1 relaxation time of the nucleus of interest and thus the necessary recycle delay. Here, we present the rationale to utilize high-spin iron(III) as the optimal transition metal for this purpose and characterize the properties of its neutral chelate form Fe(DO3A) as a suitable PRE agent. Fe(DO3A) effectively reduces the T 1 values across the entire… CONTINUE READING