Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus

  title={Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus},
  author={Enwu Liu and Jonathan E. Page},
  journal={Plant Methods},
  pages={5 - 5}
Virus-induced gene silencing (VIGS) has emerged as a method for performing rapid loss-of-function experiments in plants. Despite its expanding use, the effect of host gene insert length and other properties on silencing efficiency have not been systematically tested. In this study, we probed the optimal properties of cDNA fragments of the phytoene desaturase (PDS) gene for efficient VIGS in Nicotiana benthamiana using tobacco rattle virus (TRV). NbPDS inserts of between 192 bp and 1304 bp led… CONTINUE READING


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