Optimized Sequence Library Design for Efficient In Vitro Interaction Mapping.

@article{Orenstein2017OptimizedSL,
  title={Optimized Sequence Library Design for Efficient In Vitro Interaction Mapping.},
  author={Yaron Orenstein and Robert Puccinelli and Ryan Sangwoo Kim and Polly M Fordyce and Bonnie Berger},
  journal={Cell systems},
  year={2017},
  volume={5 3},
  pages={230-236.e5}
}
Sequence libraries that cover all k-mers enable universal, unbiased measurements of binding to both oligonucleotides and peptides. While the number of k-mers grows exponentially in k, space on all experimental platforms is limited. Here, we shrink k-mer library sizes by using joker characters, which represent all characters in the alphabet simultaneously… CONTINUE READING