Optimized Protein Extraction for Quantitative Proteomics of Yeasts

  title={Optimized Protein Extraction for Quantitative Proteomics of Yeasts},
  author={Tobias von der Haar},
  journal={PLoS ONE},
  pages={7357 - 7368}
BACKGROUND The absolute quantification of intracellular protein levels is technically demanding, but has recently become more prominent because novel approaches like systems biology and metabolic control analysis require knowledge of these parameters. Current protocols for the extraction of proteins from yeast cells are likely to introduce artifacts into quantification procedures because of incomplete or selective extraction. PRINCIPAL FINDINGS We have developed a novel procedure for protein… CONTINUE READING
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