Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production.

Abstract

Polyethyleneimine (PEI), a cationic polymer vehicle, forms a complex with DNA which then can carry anionic nucleic acids into eukaryotic cells. PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency of PEI-based transfection is affected by numerous factors, including the way the PEI/DNA complex is prepared, the ratio… (More)
DOI: 10.1002/cpch.25

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Cite this paper

@article{Yang2017OptimizedPT, title={Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production.}, author={Shaozhe Yang and Xiaoling Zhou and Rongxiang Li and Xiuhong Fu and Pingnan Sun}, journal={Current protocols in chemical biology}, year={2017}, volume={9 3}, pages={147-157} }