Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain

Abstract

Multi-polypeptide proteins such as antibodies are difficult to express in prokaryotic systems such as E. coli due to the complexity of protein folding plus secretion. Thus far, proprietary strains or fermenter cultures have been required for appreciable yields. Previous studies have shown that expression of heterologous proteins in E. coli can be enhanced… (More)
DOI: 10.1371/journal.pone.0010261

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