Optimization of virus yield as a strategy to improve rabies vaccine production by Vero cells in a bioreactor.

Abstract

To improve rabies vaccine production by Vero cells, we have developed a strategy based on high cell density culture and optimization of virus yield. We have first optimized cell growth in spinner flask using a Taguchi's L8 experimental design. We analyzed the effects of the following factors: initial glucose and glutamine concentrations, Cytodex 1… (More)

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