Optimization of transgene-mediated silencing in Phytophthora infestans and its association with small-interfering RNAs.

@article{AhFong2008OptimizationOT,
  title={Optimization of transgene-mediated silencing in Phytophthora infestans and its association with small-interfering RNAs.},
  author={Audrey M. V. Ah-Fong and Christina A Bormann-Chung and Howard S. Judelson},
  journal={Fungal genetics and biology : FG & B},
  year={2008},
  volume={45 8},
  pages={1197-205}
}
Methods for silencing genes in Phytophthora transformants have been demonstrated previously, but wide variation in effectiveness was reported in different studies. To optimize this important tool for functional genomics, we compared the abilities of sense, antisense, and hairpin transgenes introduced by protoplast, electroporation, and bombardment methods to silence the inf1 elicitin gene in Phytophthora infestans. A hairpin construct induced silencing three times more often than sense or… CONTINUE READING
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