Optimization of the single-strand conformation polymorphism (SSCP) technique for detection of point mutations.

@article{Glava1993OptimizationOT,
  title={Optimization of the single-strand conformation polymorphism (SSCP) technique for detection of point mutations.},
  author={Damjan Glava{\vc} and M. Dean},
  journal={Human mutation},
  year={1993},
  volume={2 5},
  pages={404-14}
}
The efficiency of detection of single base substitutions by single-stranded conformation polymorphism (SSCP) analysis was tested on 86 randomly distributed point mutations in a 193-bp-long DNA fragment of the mouse beta-globin gene. Multiple parameters were varied, including electrophoresis temperature, buffer concentration, gel concentration, acrylamide-to-bis-acrylamide ratio, and/or addition of different compounds to the gel matrix. Gels with a higher concentration of acrylamide and lower… CONTINUE READING

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