Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris.

  title={Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris.},
  author={Lo{\"i}c Briand and Vives. I. Roig Quilis. M. Cespedes Perez and Jean Claude Huet and Emmanuelle Danty and Claudine Masson and J. C. Pernollet},
  journal={Protein expression and purification},
  volume={15 3},
A honeybee putative general odorant-binding protein ASP2 has been expressed in the methylotrophic yeast Pichia pastoris. It was secreted into the buffered minimal medium using either the alpha-factor preprosequence with and without the Glu-Ala-Glu-Ala spacer peptide of Saccharomyces cerevisiae or its native signal peptide. Whereas ASP2 secreted using the alpha-factor preprosequence with the spacer peptide showed N-terminal heterogeneity, the recombinant protein using the two other secretion… CONTINUE READING


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The Journal of neuroscience : the official journal of the Society for Neuroscience • 1999
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