Optimization of the primary recovery of human interferon alpha2b from Escherichia coli inclusion bodies.

@article{Valente2006OptimizationOT,
  title={Optimization of the primary recovery of human interferon alpha2b from Escherichia coli inclusion bodies.},
  author={Carina Valente and Gabriel Ant{\'o}nio Amaro Monteiro and Joaquim Manuel Sampaio Cabral and Miguel Fevereiro and Duarte MF Prazeres},
  journal={Protein expression and purification},
  year={2006},
  volume={45 1},
  pages={226-34}
}
The human interferon alpha2b (hu-IFNalpha2b) gene was cloned in Escherichia coli JM109(DE3) and the recombinant protein was expressed as cytoplasmic inclusion bodies (IB). The present work discusses the recovery of hu-IFNalpha2b IB from the E. coli cells. An optimized protocol is proposed based on the sequential evaluation of recovery steps and parameters: (i) cell disruption, (ii) IB recovery and separation from cell debris, (iii) IB washing, and (iv) IB solubilization. Parameters such as hu… CONTINUE READING
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