Optimization of the expression system using galactose-inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae

@article{Choi1994OptimizationOT,
  title={Optimization of the expression system using galactose-inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae},
  author={E. Choi and J. Sohn and S. K. Rhee},
  journal={Applied Microbiology and Biotechnology},
  year={1994},
  volume={42},
  pages={587-594}
}
We have tried to optimize the galactose-inducible gene expression system for the overproduction of the potent thrombin-specific inhibitor, hirudin, in a genetically engineered yeast, Saccharomyces cerevisiae. The expression and secretion of hirudin were directed by the galactose-inducible promoter, GAL10, and the mating factor α pre-pro leader sequence. The initial hirudin expression level in shake-flask culture was 2.3 mg 1−1. Modification of the expression vector and optimization of culture… CONTINUE READING

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Gene expression and secretion of the anticoagulant hirudin in Saccharomyces cerevisiae

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