Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk.

@article{Kim2001OptimizationOT,
  title={Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk.},
  author={Chulwoo Kim and Mobin Khan and Dawn E. Morin and Walter L. Hurley and Deoki N Tripathy and Marcus E. Kehrli and Anthony O Oluoch and Ibulaimu I Kakoma},
  journal={Journal of dairy science},
  year={2001},
  volume={84 1},
  pages={74-83}
}
Staphylococcus aureus is an economically important and a major mastitis-causing pathogen that also poses food safety and antimicrobial resistance threats. Substances in mastitic milk inhibit the Taq DNA polymerase reaction (Taq PCR) making it of limited use for detecting S. aureus mastitis. In the study reported here, a set of oligonucleotide primers of 21 and 24 bases was used in Taq-PCR to amplify DNA from S. aureus (isolates from bovine mastitis). A specific amplicon of 270 bp was generated… CONTINUE READING