Optimization of the DGGE band identification method

  title={Optimization of the DGGE band identification method},
  author={Darja Ku{\vs}ar and Gorazd Avgu{\vs}tin},
  journal={Folia Microbiologica},
Denaturant gradient gel electrophoresis (DGGE) enables insight into the diversity of the studied microbial communities on the basis of separation of PCR amplification products according to their nucleotide sequence composition. However, the success of the method is accompanied by the inherent appearance of various sequence artifacts that bias the impression… CONTINUE READING