Optimization of shRNA inhibitors by variation of the terminal loop sequence.

  title={Optimization of shRNA inhibitors by variation of the terminal loop sequence.},
  author={Nick C. T. Schopman and Ying Liu and Pavlina D. Konstantinova and Olivier Ter Brake and Ben Berkhout},
  journal={Antiviral research},
  volume={86 2},
Gene silencing by RNA interference (RNAi) can be achieved by intracellular expression of a short hairpin RNA (shRNA) that is processed into the effective small interfering RNA (siRNA) inhibitor by the RNAi machinery. Previous studies indicate that shRNA molecules do not always reflect the activity of corresponding synthetic siRNAs that attack the same target sequence. One obvious difference between these two effector molecules is the hairpin loop of the shRNA. Most studies use the original… CONTINUE READING
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