Optimization of separation and digestion conditions in immune complexome analysis.

Abstract

Immune complexome analysis is a method for identifying and profiling of antigens in circulating immune complexes (CICs); it involves separation of immune complexes from serum, direct tryptic digestion of these complexes, and protein analysis via nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS). To improve this method, we initially… (More)
DOI: 10.1016/j.ab.2013.08.026

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Cite this paper

@article{Baba2013OptimizationOS, title={Optimization of separation and digestion conditions in immune complexome analysis.}, author={Miyako Baba and Kaname Ohyama and Naoya Kishikawa and Naotaka Kuroda}, journal={Analytical biochemistry}, year={2013}, volume={443 2}, pages={181-6} }