Optimization of riboflavin production by recombinant Bacillus subtilis RH44 using statistical designs


A sequential optimization strategy, based on statistical experimental designs, was used to enhance the production of riboflavin by recombinant Bacillus subtilis RH44. In the first instance, the medium components were optimized in shake flask cultures. After preliminary experiments of nitrogen source selection, the two-level Plackett–Burman (PB) design was… (More)
DOI: 10.1007/s00253-007-1049-y


12 Figures and Tables