Optimization of recombinant hyperthermophilic esterase production from agricultural waste using response surface methodology.

@article{Ren2006OptimizationOR,
  title={Optimization of recombinant hyperthermophilic esterase production from agricultural waste using response surface methodology.},
  author={Xiaodong Ren and Dawei Yu and Siping Han and Yan Feng},
  journal={Bioresource technology},
  year={2006},
  volume={97 18},
  pages={2345-9}
}
The aim of this work was to evaluate the capability of corn steep liquor being a low cost substrate on the recombinant protein production by cultivating recombinant Escherichia coli and increasing the production of hyperthermophilic esterase (HE). The effect of corn steep liquor, mineral salt and trace metals on hyperthermophilic esterase production was investigated by means of a five-level three-factor central composite rotatable design. Optimized values of the factors were determined and a… CONTINUE READING