Optimization of protoplast isolation from NaCl stressed primary, secondary and tertiary calli derived from mature seeds of Bangladeshi indica rice cultivar Binnatoa

@article{Aditya2004OptimizationOP,
  title={Optimization of protoplast isolation from NaCl stressed primary, secondary and tertiary calli derived from mature seeds of Bangladeshi indica rice cultivar Binnatoa},
  author={T. L. Aditya and David A. Baker},
  journal={Plant Growth Regulation},
  year={2004},
  volume={41},
  pages={49-56}
}
A standardized protocol was developed for the isolation of protoplasts from salt stressed primary, secondary and tertiary calli of the moderately salt tolerant indica rice land race Binnatoa. Calli were induced from mature seeds using MS2 callus induction media supplemented with 0, 50 and 100 mM NaCl. Subsequently cultures were maintained in the same medium for 1–2 passages with or without salt stress at the same concentrations. Large numbers of protoplasts (about 1.57–2.10 × 105/ml) with high… 

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