Optimization of protoplast isolation from NaCl stressed primary, secondary and tertiary calli derived from mature seeds of Bangladeshi indica rice cultivar Binnatoa

  title={Optimization of protoplast isolation from NaCl stressed primary, secondary and tertiary calli derived from mature seeds of Bangladeshi indica rice cultivar Binnatoa},
  author={T. L. Aditya and David A. Baker},
  journal={Plant Growth Regulation},
A standardized protocol was developed for the isolation of protoplasts from salt stressed primary, secondary and tertiary calli of the moderately salt tolerant indica rice land race Binnatoa. Calli were induced from mature seeds using MS2 callus induction media supplemented with 0, 50 and 100 mM NaCl. Subsequently cultures were maintained in the same medium for 1–2 passages with or without salt stress at the same concentrations. Large numbers of protoplasts (about 1.57–2.10 × 105/ml) with high… 

Figures and Tables from this paper

Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.
The protocol might encourage the plant recovery using in apple somatic hybridization programs and diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation.
Isolation and culture of protoplasts from anthers of apple (Malus pumila Mill.) rootstock ‘M9’
A reliable method for callus induction, isolation, and the culture of anther-derived protoplasts from the apple rootstock, ‘M9’ is described and information on the development of a protoplast-to-plant regeneration system is provided.
Protoplast Isolation, Salt Stress and Callus Formation of Two Date Palm Genotypes
This study reports for the first time protoplast isolation, salt stress and c allus formation from protoplasts in date palm (Phoenix dactylifera L.). Protoplasts were isolated from young leaves of
Protoplast production and isolation from Etlingera elatior
The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh matter, and the optimal incubation period was 15 hours, while greater yields were obtained using a rotatory system with protoplast incubated in the dark.
2004 SIVB Congress Symposium Proceedings “Thinking Outside the Cell”: Plant Protoplast Technology: Status and Applications
SummaryPlant protoplasts provide an enabling technology to underpin aspects of development, physiology, and genetics. Reliable procedures are available to isolate and culture protoplasts from


Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars
A plant regeneration system from rice protoplasts using calli derived from mature embryos was established for the two Brazilian modern rice cultivars Iac-201 and IAC-165 and showed morphological variation such as the presence of awns in spite of the short time of the in vitro culture.
Fertile Indica rice plants regenerated from protoplasts isolated from scutellar tissue of immature embryos
All scutellum protoplast-derived plants that were transferred to the greenhouse survived and were fertile and a more effective media was found to be more effective in producing green plants.
An improved procedure for plant regeneration from indica and japonica rice protoplasts
In the japonica rice variety Taipei 309, this new procedure resulted in a 30-fold increase in plant regeneration from protoplasts compared to previous published procedures.
Plant Regeneration from Mesophyll Protoplasts of Rice (Oryza sativa L.)
The results show that contrary to popular belief, mesophyll protoplasts of rice and perhaps other gramineous plants are capable of dedifferentiation and re-entry into the cell cycle, and are totipotent.
Plant regeneration from indica rice (Oryza sativa L.) protoplasts
Two other suspension cultures of IR54, one developed from the protoplast callus of the initial IR54 line, and the other developed from callus produced by mature seeds, have yielded protoplasts capable of regenerating plants when using cells of the Calrose 76 suspension as a nurse culture.
Fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures
A system to regenerate fertile rice (Oryza sativa L.) plants from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established and green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiation.
Intergeneric somatic hybrids of rice [Oryza sativa L. (+) Porteresia coarctata (Roxb.) Tateoka]
Plants of one line had an allohexaploid chromosome complement and, following examination of its vegetative clones by GISH, were confirmed as somatic hybrids containing full chromosome complements of both O. sativa and P. coarctata.
Transgenic Indica rice (Oryza sativa L.) plants obtained by direct gene transfer to protoplasts.