Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

@inproceedings{Jafari2011OptimizationOP,
  title={Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments},
  author={Rozbeh Jafari and Birgitta E Sundstr{\"o}m and Patrik Holm},
  booktitle={Microbial cell factories},
  year={2011}
}
BACKGROUND Optimization of conditions during recombinant protein production for improved yield is a major goal for protein scientists. Typically this is achieved by changing single crucial factor settings one at a time while other factors are kept fixed through trial-and-error experimentation. This approach may introduce larger bias and fail to identify interactions between the factors resulting in failure of finding the true optimal conditions. RESULTS In this study we have utilized design… CONTINUE READING