Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.

@article{Parashar2004OptimizationOP,
  title={Optimization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India.},
  author={Deepti Parashar and Devendra Singh Chauhan and V. D. Sharma and Aradhana Chauhan and S. V. S. Chauhan and Vishwa Mohan Katoch},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 6},
  pages={3751-3}
}
For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.