Optimization of primer design for the detection of variable genomic lesions in cancer

  title={Optimization of primer design for the detection of variable genomic lesions in cancer},
  author={Ali Bashir and Yu-Tsueng Liu and Benjamin J. Raphael and Dennis Carson and Vineet Bafna},
  volume={23 21},
Primer approximation multiplex PCR (PAMP) is a new experimental protocol for efficiently assaying structural variation in genomes. PAMP is particularly suited to cancer genomes where the precise breakpoints of alterations such as deletions or translocations vary between patients. The design of PCR primer sets for PAMP is challenging because a large number of primer pairs are required to detect alterations in the hundreds of kilobases range that can occur in cancer. These sets of primers must… CONTINUE READING


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