Optimization of primer design for the detection of variable genomic lesions in cancer

@article{Bashir2007OptimizationOP,
  title={Optimization of primer design for the detection of variable genomic lesions in cancer},
  author={Ali Bashir and Yu-Tsueng Liu and Benjamin J. Raphael and Dennis Carson and Vineet Bafna},
  journal={Bioinformatics},
  year={2007},
  volume={23 21},
  pages={2807-15}
}
Primer approximation multiplex PCR (PAMP) is a new experimental protocol for efficiently assaying structural variation in genomes. PAMP is particularly suited to cancer genomes where the precise breakpoints of alterations such as deletions or translocations vary between patients. The design of PCR primer sets for PAMP is challenging because a large number of primer pairs are required to detect alterations in the hundreds of kilobases range that can occur in cancer. These sets of primers must… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

Optimizing PCR Assays for DNA-Based Cancer Diagnostics

Journal of Computational Biology • 2010
View 3 Excerpts

References

Publications referenced by this paper.
Showing 1-10 of 29 references

A novel approach for determining cancer genomic breakpoints in the presence of normal DNA

Liu, Y.-T, D. A. Carson
PLoS ONE, • 2007
View 3 Excerpts
Highly Influenced

Hardness of PAMP

J. Chuzhoy
Personal Communication • 2007
View 1 Excerpt

Highly parallel genomic assays

Nature Reviews Genetics • 2006
View 1 Excerpt

Similar Papers

Loading similar papers…