• Chemistry
  • Published 2011

Optimization of particle bombardment conditions by β- glucuronidase (GUS) reporter system in tomato fruit

@inproceedings{Sun2011OptimizationOP,
  title={Optimization of particle bombardment conditions by β- glucuronidase (GUS) reporter system in tomato fruit},
  author={Liang Sun and Shutong Liu and Jie Ren and Mengmeng Cui and Ling D. Wang and Ping Leng},
  year={2011}
}
Fruits of tomato cultivar R-144 ( Lycopersicon esculentum Mill. cv. R-144), a variety from Israel, were bombarded on tungsten particles coated with a plasmid containing GusPlus gene that was coded for βglucuronidase (GUS). Peels of target areas of fruits were removed before bombardment, and as such, after 24 h, the effects of different bombardment parameters were evaluated by comparing the numbers of blue spots which resulted to histological GUS assays. The effects of bombardment pressure… CONTINUE READING

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