Optimization of organic solvent in multiphase biocatalysis.

@article{Brink1985OptimizationOO,
  title={Optimization of organic solvent in multiphase biocatalysis.},
  author={L. E. S. Brink and Johannes Tramper},
  journal={Biotechnology and bioengineering},
  year={1985},
  volume={27 8},
  pages={1258-69}
}
The microbial epoxidation of propene and 1-butene was used to study some fundamental aspects of two-liquid-phase biocatalytic conversions. Introduction of a water-immiscible organic solvent phase in a free-cell suspension gave rise to a series of undesired phenomena, e.g., inactivation by the solvent, clotting of biomass, and aggregation of cells at the liquid-liquid interface. Immobilization of the cells in hydrophilic gels, e.g., calcium alginate, prevented direct cell-organic solvent contact… CONTINUE READING

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