Optimization of methods for detecting norovirus on various fruit.

  title={Optimization of methods for detecting norovirus on various fruit.},
  author={Hee-Yeon Kim and I. Kwak and In Gyun Hwang and Gwangpyo Ko},
  journal={Journal of virological methods},
  volume={153 2},
Methods for detecting norovirus (NoV) in food are crucial for investigation and prevention of outbreaks caused by NoV-contaminated food. However, current NoV detection methods have not been well examined or optimized. In this study, the effectiveness of various methods for eluting NoV from various fruit, concentrating the virus using polyethylene glycol (PEG), and extracting the viral RNA for subsequent assay by RT-PCR was optimized. First, six different buffers previously described for eluting… CONTINUE READING


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