Optimization of mesenchymal stem cell expansion procedures by cell separation and culture conditions modification.

@article{Carrancio2008OptimizationOM,
  title={Optimization of mesenchymal stem cell expansion procedures by cell separation and culture conditions modification.},
  author={Soraya Carrancio and Natalia L{\'o}pez-Holgado and Ferm{\'i}n S{\'a}nchez-Guijo and Eva Mar{\'i}a Villar{\'o}n and Victoria Barbado and Soraya Tabera and Mar{\'i}a D{\'i}ez-Campelo and Juan Zapata Blanco and Jes{\'u}s Fernando San Miguel and Mar{\'i}a Consuelo del Ca{\~n}izo},
  journal={Experimental hematology},
  year={2008},
  volume={36 8},
  pages={1014-21}
}
OBJECTIVE Optimization of the mesenchymal stem cells (MSC) isolation and expansion method. MATERIALS AND METHODS Mononuclear cells (MNC) from bone marrow aspirates were obtained by both density gradient centrifugation (standard method) and gravity sedimentation. Cells were cultured in standard conditions (10% fetal calf serum and normal oxygen tension [21% O(2)]) and expansion results compared to those obtained with the same culture conditions to which platelet lysate (PL) preparations were… CONTINUE READING
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