Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture

  title={Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture},
  author={S. Pointing and E. Jones and L. Vrijmoed},
The white-rot fungus Pycnoporus sanguineus produces laccase under a range of C/N ratios in submerged liquid culture. Enzyme production was increased 50 fold in the presence of 20 μM xylidine to a m... 
Extracellular laccase activity in Tetraploa aristata
Tetraploa aristata CLPS 419 produced maximum extracellular laccase activity at over 9 mU ml−1 in shaking cultures supplemented with glucose and 3.5mU ml+1 in sucrose-grown ones, and was not detected in similar cultures with sucrose or in ones grown on lignin. Expand
Purification of Intracellular Laccase from Schizophyllum sp. Isolated from Forest Source
Laccase enzymes extensively produced by white-rot fungi are evaluated in the field of biotechnology research due to its unique properties such as high reaction rates, broad substrate specificity andExpand
Properties of Laccases Produced by Pycnoporus sanguineus Induced by 2,5-xylidine
Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography and sodium azide inhibited Lac I and Lac II activities. Expand
Enhancement of laccase production by Pleurotus ostreatus and its use for the decolorization of anthraquinone dye
Abstract The white-rot fungus Pleurotus ostreatus strain 32 is an excellent producer of the industrially important enzyme laccase. Laccase was the only ligninolytic activity detected in theExpand
Modeling of growth and laccase production by Pycnoporus sanguineus
Production of extracellular laccase by the white-rot fungus Pycnoporus sanguineus was examined in batch submerged cultures in shake flasks, baffledshake flasks and a stirred tank bioreactor, and a modified Luedeking-Piret model incorporating logistic growth effectively described the consumption of glucose. Expand
Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design
The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducersExpand
Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor
Laccase production by T. multicolor decreased considerably when the fungus was grown in a stirred-tank reactor, presumably because of damage of the mycelia caused by shear stress and/or changes in the morphology of the fungus. Expand
Laccase production using mixed substrates containing lignocellulosic materials by Pleurotus ostreatus in submerged liquid culture
Enhanced production of the industrially important enzyme laccase from the white rot fungus Pleurotus ostreatus MTCC1804 was performed in the submerged liquid culture. The preliminary media componentExpand
Optimization of Laccase Production from Penicillium martensii
Laccase production by Penicillium martensii NRC 345 was optimized. Eight media with different components were screened. The enzyme formed by P. martensii NRC 345 was detected mainly exocellularlyExpand
Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens
By applying a fed-batch strategy, in which a glucose solution was fed continuously to a cultivation of T. pubescens so that the glucose concentration in the medium never exceeded a certain low, critical value, glucose repression could be avoided and production of laccase was almost doubled as compared to the batch cultivation. Expand


Role of a Laccase in the Degradation of Pentachlorophenol
The ability to mineralize organic pollutants in vitro is generally enhanced under culture conditions favorable to the mineralization of the lignin model compounds which suggests that the lIGNin-degrading system may be involved in the transformation of anthropogenic compounds. Expand
Phenoloxidases and hydrolases from Pycnoporus sanguineus (UEC-2050 strain): applications
This fungus was efficient not only in chromophore group transformations but also in the reduction of phenols, biochemical and chemical oxygen demands, and toxicity. Expand
Laccase is essential for lignin degradation by the white‐rot fungus Pycnoporus cinnabarinus
It is shown that laccase‐less mutants of P. cinnabarinus were greatly reduced in their ability to metabolize 14C ring‐labeled DHP, but 14CO2 evolution in these mutant cultures could be restored to levels comparable to those of the wild‐type cultures by addition of purified P.cinnabaranus laccases. Expand
The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase
The white rot fungus Pycnoporus cinnabarinus was characterized with respect to its set of extracellular phenoloxidases and a single predominant laccase and a lack of lignin- or manganese-type peroxidase make this organism an interesting model for further studies of possible alternative pathways of ligningin degradation by white rot fungi. Expand
Laccase, cellulase and xylanase activities during growth ofPleurotus sajor-caju on sagohampas
Sagohampas, the fibrous pith residue left after starch extraction from sago palm, is abundant at sago-processing factories and can be used as a substrate for the production of laccase by solidExpand
Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767
Laccase activity was observed even after addition of excess catalase to the extracellular culture fluid to destroy the endogenously produced hydrogen peroxide, indicating that the observed activity is not due to a peroxidase. Expand
Purification and Properties of Laccase Excreted by Pycnoporus coccineus
er was replaced with 0.1 m K phosphate buffer (KPB), pH 7.0. Protein in the eluate was monitored by absorbance at 280nm. Pycnoporus coccineus IFO-4923 was cultured in the mediumof Fahraeus andExpand
Lignocellulolytic enzyme profiles of edible mushroom fungi
Examination of the lignocellulolytic enzyme profiles of the three important commercially cultivated mushrooms show diversity to be reflected in qualitative variations in the major enzymic determinants required for substrate bioconversion. Expand
The potential for white-rot fungi in the treatment of pollutants
Abstract Lignin-degrading white-rot fungi have the unique ability to degrade/mineralize a broad spectrum of structurally diverse toxic environmental pollutants. Extracellular peroxidases areExpand
Comparison of wood decay among diverse lignicolous fungi
In decay tests with 98 isolates (78 species) of lignicolous fungi followed by chemical and anatomical analyses, the validity of the generally accepted, major decay types (white, brown, and soft rot...