Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA.

@article{Freitas2006OptimizationOI,
  title={Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA.},
  author={Sind{\'e}lia S Freitas and Jose L. Santos and D M F Prazeres},
  journal={Biotechnology progress},
  year={2006},
  volume={22 4},
  pages={1179-86}
}
Large-scale processes used to manufacture grams of plasmid DNA (pDNA) should be cGMP compliant, economically feasible, and environmentally friendly. Alcohol and salt precipitation techniques are frequently used in plasmid DNA (pDNA) downstream processing, as concentration and prepurification steps, respectively. This work describes a study of a standard 2-propanol (IsopOH; 0.7 v/v) and ammonium sulfate (AS; 2.5 M) precipitation. When inserted in a full process, this tandem precipitation scheme… CONTINUE READING