Optimization of intracellular microcystin-LR extraction for its analysis by protein phosphatase inhibition assay.

@article{Sevilla2009OptimizationOI,
  title={Optimization of intracellular microcystin-LR extraction for its analysis by protein phosphatase inhibition assay.},
  author={Emma Sevilla and Henry G. F. Smienk and Pedro Razquin and Luis Mata and Mar{\'i}a Luisa Peleato},
  journal={Water science and technology : a journal of the International Association on Water Pollution Research},
  year={2009},
  volume={60 7},
  pages={1903-9}
}
Microcystins are toxins produced by some strains of cyanobacteria. Several methods have been developed to allow the quantification of microcystins, which are mainly endotoxins. Among those methods, the protein phosphatase inhibition assay is a good candidate as a screening method because of its sensitivity, simplicity and specificity. In this work a method for intracellular microcystin extraction in field water samples and lab cyanobacterial cultures prior to their analysis by protein… CONTINUE READING

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