Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques

  title={Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques},
  author={Karsten Hellmuth and S. Pluschkell and J Jung and E F Ruttkowski and Ursula Rinas},
  journal={Applied Microbiology and Biotechnology},
Wild-type Aspergillus niger NRRL-3 was transformed with multiple copies of the glucose oxidase structural gene (god). The gene was placed under the control of the gpd A promoter of A. nidulans. For more efficient secretion the α-amylase signal peptide from A oryzae was inserted in front of god. Compared to the wild type, the recombinant strain NRRL-3 (GOD3-18) produced up to four times more extracellular glucose oxidase under identical culture conditions. Addition of yeast extract (2 g l−1) to… CONTINUE READING
11 Citations
30 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 11 extracted citations


Publications referenced by this paper.
Showing 1-10 of 30 references

Ubicon - ein dezentrales Prozegleitsystem fiir die Biotechnologie

  • K-H Bellgardt, T Gfibel, +4 authors W Schulze
  • Bioengineering
  • 1992
1 Excerpt

duction of extracellular proteins by the filamentous fungus Aspergillus

  • PJ Punt, Gorcom RFM van
  • Antonie Van Leeuvenhoek
  • 1992

Contribution of endo - and exocellular glucose oxidase to gluconic acid production at increased dissolved oxygen concentrations

  • M Traeger, GN Qazi, U Onken, CL Chopra
  • J Chem Technol Biotechnol
  • 1991

Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed AspergiIlus nidulans 9 pdA gene

  • J PuntP, ND Zegers, M Busscher, PH Powels, Hondel CAMJJ van den
  • J Biotechnol
  • 1991

Functional elements

  • PH, Hondel CAMJJ van den
  • 1990

Similar Papers

Loading similar papers…