Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques

@article{Hellmuth1995OptimizationOG,
  title={Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques},
  author={Karsten Hellmuth and S. Pluschkell and J Jung and E F Ruttkowski and Ursula Rinas},
  journal={Applied Microbiology and Biotechnology},
  year={1995},
  volume={43},
  pages={978-984}
}
Wild-type Aspergillus niger NRRL-3 was transformed with multiple copies of the glucose oxidase structural gene (god). The gene was placed under the control of the gpd A promoter of A. nidulans. For more efficient secretion the α-amylase signal peptide from A oryzae was inserted in front of god. Compared to the wild type, the recombinant strain NRRL-3 (GOD3-18) produced up to four times more extracellular glucose oxidase under identical culture conditions. Addition of yeast extract (2 g l−1) to… CONTINUE READING
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