Optimization of fermentation conditions for preparation of polygalacturonic acid transeliminase by Erwinia carotovora IFO3830.

@article{Ding2001OptimizationOF,
  title={Optimization of fermentation conditions for preparation of polygalacturonic acid transeliminase by Erwinia carotovora IFO3830.},
  author={Fuli Ding and Hidetaka Noritomi and Koji Nagahama},
  journal={Biotechnology progress},
  year={2001},
  volume={17 2},
  pages={311-7}
}
This paper is concerned with optimization of the fermentation conditions for preparation of alkaline pectin lyase (poly-galacturonic acid trans-eliminase, PATE) by Erwinia carotovora IFO3830 (E.C.IFO3830). The orthogonal matrix method was adopted as the experimental design method for media. The effects of media composition on the growth rate of E.C.IFO3830 are in the order of pectin > KH(2)PO(4)/Na(2)HPO(4) > MgSO(4) > (NH(4))(2)SO(4) > glucose > L-glutamate sodium, and those on PATE activity… CONTINUE READING