Optimization of fed-batch production of the model recombinant protein GFP in Lactococcus lactis.

@article{Oddone2007OptimizationOF,
  title={Optimization of fed-batch production of the model recombinant protein GFP in Lactococcus lactis.},
  author={Gian M Oddone and Christopher Q Lan and Helen Rawsthorne and David A. Mills and David E. Block},
  journal={Biotechnology and bioengineering},
  year={2007},
  volume={96 6},
  pages={1127-38}
}
Optimization of recombinant protein production using lactic acid bacteria (LAB) remains an important obstacle on the road to realizing LAB as oral vaccine delivery vehicles. Despite this, there have been few published investigations to explore the higher limits of LAB recombinant protein expression in fed-batch fermentations. In this study, results from response surface experiments suggested an optimal set of conditions for expression of green fluorescent protein (GFP), a model recombinant… CONTINUE READING

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