Optimization of estrogen growth response in MCF-7 cells

  title={Optimization of estrogen growth response in MCF-7 cells},
  author={Thomas E. Wiese and Leos G. Kral and Kathleen E. Dennis and W. Barkley Butler and Samuel Carroll Brooks},
  journal={In Vitro Cellular & Developmental Biology - Animal},
The factors involved in estradiol-17β induced growth stimulation of MCF-7 human breast cancer cells have been examined. Wild type MCF-7 cells (and clone E3) were shown to undergo slow growth in phenol-red-free medium containing specific calf sera. The E3 clone was used to document a mean 6-day growth stimulation of 3.35-fold (doubling time=33±3 h) in cultures supplemented with 10−11 M estradiol-17β. The serum batch utilized in the culture medium is most important in acquiring significant growth… CONTINUE READING


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