Optimization of embryo culture conditions for increasing efficiency of cloning in buffalo (Bubalus bubalis) and generation of transgenic embryos via cloning.

Abstract

Cloning in bovine species is marred by low efficiency of blastocyst formation. Any increase in the efficiency of blastocyst formation upon nuclear transfer will greatly enhance the efficiency of cloning. In the present study, the effect of various media, protein sources, and growth factors on the development of cloned buffalo embryos was evaluated. Among… (More)
DOI: 10.1089/clo.2009.0003

Topics

Cite this paper

@article{Wadhwa2009OptimizationOE, title={Optimization of embryo culture conditions for increasing efficiency of cloning in buffalo (Bubalus bubalis) and generation of transgenic embryos via cloning.}, author={Neerja Wadhwa and Neetu Kunj and Shuchita Tiwari and Megha Saraiya and Subeer S Majumdar}, journal={Cloning and stem cells}, year={2009}, volume={11 3}, pages={387-95} }