Optimization of differential immunogold-silver and peroxidase labeling with maintenance of ultrastructure in brain sections before plastic embedding.

Abstract

The limited success of immunogold labeling for pre-embedding immunocytochemistry of neuronal antigens is largely attributed to poor penetration of large (5-20 nm) colloidal gold particles. We examined the applicability of using silver intensification of 1 nm colloidal gold particles non-covalently bound to goat anti-rabbit immunoglobulin (1) for single… (More)

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Cite this paper

@article{Chan1990OptimizationOD, title={Optimization of differential immunogold-silver and peroxidase labeling with maintenance of ultrastructure in brain sections before plastic embedding.}, author={John K. Chan and Chiye J Aoki and Virginia M Pickel}, journal={Journal of neuroscience methods}, year={1990}, volume={33 2-3}, pages={113-27} }