Optimization of delivery of foreign DNA into higher-plant chloroplasts

@article{Ye1990OptimizationOD,
  title={Optimization of delivery of foreign DNA into higher-plant chloroplasts},
  author={G N Ye and Henry Daniell and John C. Sanford},
  journal={Plant Molecular Biology},
  year={1990},
  volume={15},
  pages={809-819}
}
We report here an efficient and highly reproducible delivery system, using an improved biolistic transformation device, that facilitates transient expression of β-glucuronidase (GUS) in chloroplasts of cultured tobacco suspension cells. Cultured tobacco cells collected on filter papers were bombarded with tungsten particles coated with pUC118 or pBI101.3 (negative controls), pBI505 (positive nuclear control) or a chloroplast expression vector (pHD203-GUS), and were assayed for GUS activity. No… CONTINUE READING

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