Optimization of culture conditions for production of cis-epoxysuccinic acid hydrolase using response surface methodology.

@article{Li2008OptimizationOC,
  title={Optimization of culture conditions for production of cis-epoxysuccinic acid hydrolase using response surface methodology.},
  author={Xia Li and Tongcheng Xu and Xiaohang Ma and Kangping Guo and Lei Kai and Yuhua Zhao and Xiaoming Jia and Yin Shan Ma},
  journal={Bioresource technology},
  year={2008},
  volume={99 13},
  pages={5391-6}
}
Response surface methodology, which allows for rapid identification of important factors and optimization of them to enhance enzyme production, was employed here to optimize culture conditions for the production of cis-epoxysuccinic acid hydrolase from Bordetella sp. strain 1-3. In the first step, a Plackett-Burman design was used to evaluate the effects of nine variables (yeast extract, cis-epoxysuccinic acid, KH(2)PO(4), K(2)HPO(4).3H(2)O, MgSO(4).7H(2)O, trace minerals solution, culture… CONTINUE READING