Optimization of culture and storage conditions for an in vitro system to evaluate thymocyte phenotype and function.

@article{Young2006OptimizationOC,
  title={Optimization of culture and storage conditions for an in vitro system to evaluate thymocyte phenotype and function.},
  author={Charlene D Young and Jonathan B Angel},
  journal={Journal of immunological methods},
  year={2006},
  volume={312 1-2},
  pages={
          157-66
        }
}
Studies on thymopoiesis are critical to the understanding of T-cell homeostasis as well as the host response to T-cell depletion. Various in vitro culture systems have been used in the study of thymocyte development; however it is unclear if current co-culture methods have been fully optimized. In this study in vitro suspension cultures have been re-evaluated and the optimal storage conditions for thymocytes have been established by evaluating various methods of storing/isolating thymic tissue… CONTINUE READING