Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens

@inproceedings{Somfai2015OptimizationOC,
  title={Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed
porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration
regimens},
  author={T Somfai and Nguyen Thi Men and Junko Noguchi and Hiroyuki Kaneko and Naomi Kashiwazaki and Kazuhiro Kikuchi},
  booktitle={The Journal of reproduction and development},
  year={2015}
}
Our aim was to optimize the cryoprotectant treatment for the preservation of immature porcine cumulus-oocyte complexes (COCs) by solid surface vitrification. In each experiment, the vitrification solution consisted of 50 mg/ml polyvinyl pyrrolidone, 0.3 M of the actual sugar and in total 35% (v/v) of the actual permeating cryoprotectant (pCPA) combination. After warming, the COCs were subjected to in vitro maturation, fertilization and embryo culture. In Experiment 1, trehalose and sucrose were… CONTINUE READING

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