Optimization of conditions for profiling bacterial populations in food by culture-independent methods.

@article{Cocolin2007OptimizationOC,
  title={Optimization of conditions for profiling bacterial populations in food by culture-independent methods.},
  author={Luca Cocolin and Ana Laura Diez and Rosalinda Urso and Kalliopi Rantsiou and Giuseppe Comi and Ingrid Bergmaier and Claudia Beimfohr},
  journal={International journal of food microbiology},
  year={2007},
  volume={120 1-2},
  pages={100-9}
}
In this study we used culture-independent methods to profile bacterial populations in food products. Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH) were employed in order to identify bacterial species without the need of isolation and biochemical identification. The protocols used to extract the DNA, subsequently subjected to PCR amplification for DGGE, as well as the hybridization procedure for FISH, were optimised. Moreover, an extensive study on… CONTINUE READING

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