Optimization of conditions for growth of wild-type and genetically transformed Trypanosoma cruzi on agarose plates.

@article{Mondragon1999OptimizationOC,
  title={Optimization of conditions for growth of wild-type and genetically transformed Trypanosoma cruzi on agarose plates.},
  author={Alberto Mondragon and Shane R. Wilkinson and Martin Craig Taylor and John M. Kelly},
  journal={Parasitology},
  year={1999},
  volume={118 ( Pt 5)},
  pages={461-7}
}
Growth of Trypanosoma cruzi as colonies on solid medium has not been widely used as an experimental procedure. We therefore sought to establish a reliable and routine plating method. The optimal results were achieved with a matrix of 0.65% low melting point agarose onto which epimasigotes from the mid-to-late logarithmic phase of growth were spread. Colonies could be isolated after incubation for 21 days in a humidified 5% CO2 environment at 28 degrees C. Plating efficiencies in the range of 40… CONTINUE READING