Optimization of buffer solutions for protein crystallization.

  title={Optimization of buffer solutions for protein crystallization.},
  author={Rajendrakumar A Gosavi and Timothy C. Mueser and Constance A Schall},
  journal={Acta crystallographica. Section D, Biological crystallography},
  volume={64 Pt 5},
Increasing the solubility of protein stock solutions to above that in a standard chromatography buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl) led to an increase in the number of crystallization conditions for ten globular proteins subjected to two crystal screens: the Index and Precipitant/Precipitant-Additive (P/PA) Screens. Solubility enhancement of protein stock solutions was achieved through screening and selection of buffer components to formulate an optimal buffer. Relative improvements in… CONTINUE READING

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