Corpus ID: 88735678

Optimization of antibody responses of a malaria DNA vaccine in Aotus monkeys

  title={Optimization of antibody responses of a malaria DNA vaccine in Aotus monkeys},
  author={Robert A Gramzinski and D. C. Maris and Nicanor Obald{\'i}a and Richard N. Rossan and Martha Sedegah and Ruobing Wang and Peter M. Hobart and Michal Margalith and Stephen L. Hoffman},
Effect of nanoparticle coating on the immunogenicity of plasmid DNA vaccine encoding P. yoelii MSP-1 C-terminal.
The results overall indicate the possibility of selection and optimization of DNA vaccine formulation for intravenous delivery and may be useful in designing a nanoparticle-coated DNA vaccine that could optimally elicit a desired antibody response for various disease conditions. Expand
Adaptation of a Thai multidrug-resistant C2A clone of Plasmodium falciparum to Aotus monkeys and its preliminary in vivo antimalarial drug efficacy-resistance profile.
A multidrug-resistant (MDR) clone of Plasmodium falciparum from Thailand was adapted through serial passage to Aotus monkeys, and showed resistance to a single 20 or 40 mg/kg oral dose of mefloquine. Expand
DNA Vaccines for Mucosal Immunity to Infectious Diseases
This chapter discusses mucosal immunity primarily in the context of DNA vaccination against infectious agents, and it may be that epidermal inoculation is as effective in inducing immunity at distal mucosal sites as that obtained by direct mucosal immunization. Expand
The development of a multivalent DNA vaccine for malaria
According to the latest report from the World Health Organization (December 1996), malaria is still a public health problem in more than 90 countries, inhabited by a total of some 2.4 billion peopleExpand
Safety, tolerability and humoral immune responses after intramuscular administration of a malaria DNA vaccine to healthy adult volunteers.
Despite induction of excellent CTL responses, intramuscular DNA vaccination via needle injection failed to induce detectable antigen-specific antibodies in any of the volunteers. Expand
Preclinical evaluation of a synthetic Plasmodium falciparum MAP malaria vaccine in Aotus monkeys and mice.
The addition of a co-adjuvant QS-21, a purified saponin, significantly increased the immunogenicity of the alum-adsorbed MAP in both mice and monkeys, providing a vaccine formulation suitable for phase I trials in human volunteers. Expand
DNA vaccination as an approach to malaria control: current status and strategies.
During the twentieth century, the primary approach to malaria prevention has been to interfere with transmission of the parasite between the infected mosquito and the human host using physicalExpand
Immune Response to a Hepatitis B DNA Vaccine in Aotus Monkeys: A Comparison of Vaccine Formulation, Route, and Method of Administration
For immunization of primates with DNA vaccines, ID may be a preferable route to IM, although it is not clear whether the Aotus monkey is a relevant model for humans in this respect. Expand
Simultaneous Induction of Multiple Antigen-Specific Cytotoxic T Lymphocytes in Nonhuman Primates by Immunization with a Mixture of Four Plasmodium falciparum DNA Plasmids
By providing the first evidence for primates that immunization with a mixture of DNA plasmids induces CD8+ T-cell responses against all the components of the mixture, these studies provide the foundation for multigene immunization of humans. Expand
Multi-gene vaccination against malaria: A multistage, multi-immune response approach.
The rationale for developing a multistage, multivalent, multi-immune response malaria vaccine is presented and why, among currently available technologies, DNA vaccines may offer the best prospect for success. Expand