Optimization of a single-chain antibody fragment overexpression in Escherichia coli using response surface methodology

Abstract

Human epidermal growth factor receptor (HER) family plays an important role in various types of cancers. As a result, antibodies against HER and the mechanism of antigen-antibody binding action are under active investigation. We previously constructed a single-chain variable fragment (ScFv) against HER2, i.e. anti-Her2 ScFv, for expressing in the… (More)

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Cite this paper

@inproceedings{Akbari2015OptimizationOA, title={Optimization of a single-chain antibody fragment overexpression in Escherichia coli using response surface methodology}, author={Vajihe Akbari and H. Mir Mohammad Sadeghi and Abbas Jafarian-dehkordi and C Perry Chou and Daryoush Abedi}, booktitle={Research in pharmaceutical sciences}, year={2015} }