Optimization of a protein extraction technique for fungal proteomics

  title={Optimization of a protein extraction technique for fungal proteomics},
  author={Vijai Bhadauria and You-Liang Peng},
  journal={Indian Journal of Microbiology},
Protein extraction is a critical step in any proteomics study. Since most fungi possess a robust cell wall, efficient isolation of total proteins has become challenge to fungal proteomics. To circumvent this bottleneck of fungal proteomics, we standardized a protocol named as Mg/CHAPS extraction by comparing with an established method of protein extraction (Tris/EDTA extraction), using 2-DE and MALDI-TOF MS. Total mycelial proteins were isolated using both protocols from Magnaporthe grisea… CONTINUE READING