Optimization of a heterologous mevalonate pathway through the use of variant HMG-CoA reductases.

@article{Ma2011OptimizationOA,
  title={Optimization of a heterologous mevalonate pathway through the use of variant HMG-CoA reductases.},
  author={Suzanne M Ma and David E. Garcia and Alyssa M. Redding-Johanson and Gregory Friedland and R. Isaac Chan and Tanveer Singh Batth and John R. Haliburton and Dylan Chivian and Jay D Keasling and Christopher J. Petzold and Taek S Lee and Swapnil R. Chhabra},
  journal={Metabolic engineering},
  year={2011},
  volume={13 5},
  pages={588-97}
}
Expression of foreign pathways often results in suboptimal performance due to unintended factors such as introduction of toxic metabolites, cofactor imbalances or poor expression of pathway components. In this study we report a 120% improvement in the production of the isoprenoid-derived sesquiterpene, amorphadiene, produced by an engineered strain of Escherichia coli developed to express the native seven-gene mevalonate pathway from Saccharomyces cerevisiae (Martin et al. 2003). This… CONTINUE READING