Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana.

@article{Hart2009OptimizationOA,
  title={Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana.},
  author={Shanion M Hart and Chhandak Basu},
  journal={Journal of biomolecular techniques : JBT},
  year={2009},
  volume={20 2},
  pages={96-100}
}
Digoxigenin is derived from a plant steroid hormone digoxin found in the plants Digitalis sp. Digoxigenin has been used successfully in labeling nucleic acids. In this experiment we optimized minimum probe requirement for a nonradioactive digoxigenin-based gene detection system in the model plant Arabidopsis thaliana. We showed that 1 microL of labeled… CONTINUE READING