Optimization of Process Parameters for Alkaline Protease Production by Bacillus licheniformis N-2 and Kinetics Studies in Batch Fermentation

@inproceedings{Nadeem2008OptimizationOP,
  title={Optimization of Process Parameters for Alkaline Protease Production by Bacillus licheniformis N-2 and Kinetics Studies in Batch Fermentation},
  author={Muhammad Nadeem and Javed Iqbal Qazi and Quratulain Syed and Shahjahan Baig},
  year={2008}
}
Production of alkaline protease from B. licheniformis N-2 was studied in shake flask experiments in growth medium comprised of (g/l): glucose, 10; soybean meal, 10; K2HPO4, 5; MgSO4.7H2O, 0.5; NaCl, 0.5; CaCl2.2H2O, 0.5. Different process parameters, such as fermentation period, initial pH, incubation temperature, agitation speed, and size and age of inoculum, were optimized for the maximum yield of the alkaline protease. Maximum enzyme production (991.04 PU/ml) was obtained after 24 h of… CONTINUE READING

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